ВЛИЯНИЕ МОЛЕКУЛЯРНОГО ВОДОРОДА НА ВЫХОД БИОАКТИВНЫХ ВЕЩЕСТВ ИЗ КОЖУРЫ ВИНОГРАДА

Abstract

In this study, the efficiency of extracting valuable compounds from grape skin was investigated using four types of solvents: conventional, H₂-saturated, acidified, and acidified and saturated with molecular hydrogen (H₂). The samples were analyzed for total phenolic content (TPC), total flavonoid content (TFC), total monomeric anthocyanins (TMA), and antioxidant activity using DPPH, ABTS, and FRAP assays. No significant differences were observed between the studied solvents in aqueous extracts. In ethanolic systems, the combination of acidification and hydrogen saturation led to a statistically significant increase in TPC and TMA contents, as well as in antioxidant activity measured by DPPH and ABTS methods. At the same time, the FRAP values remained almost unchanged, indicating the selective nature of molecular hydrogen’s effect: it primarily enhances the extraction of polyphenols and anthocyanins but does not affect the reducing capacity assessed by the FRAP method. The obtained results confirm the promise of using acidified ethanolic solvents saturated with molecular hydrogen for the targeted and efficient extraction of valuable biologically active compounds from grape processing waste.