ВЛИЯНИЕ ПРЕДВАРИТЕЛЬНОЙ ОБРАБОТКИ ПИВНОЙ ДРОБИНЫ НА ПАРАМЕТРЫ ФЕРМЕНТАЦИИ И ПРОДУКЦИЮ ВОДОРОДА

Abstract

This study explores the potential use of Brewer’s Spent Grain (BSG) as a feedstock for renewable fuel production, including molecular hydrogen and bioethanol. An optimized pretreatment of the substrate with diluted sulfuric acid was performed to maximize carbohydrate release. The liberated sugars were utilized in microbial fermentation involving Escherichia coli BW25113 wild-type and the sevenfold mutant BW25113hyaBhybChycAfdoGldhAfrdCaceE. Based on the experimental data, the optimal hydrolysis and fermentation parameters were determined, including bsg concentration (4%), acid pretreatment conditions (130℃, 1.5% H₂SO₄, 26 min), ph range (5.19-5.27), and redox potential (below –400 mV). The results demonstrated that the mutant E. coli strain is highly efficient in hydrogen production, whereas the wild-type strain promotes bacterial biomass growth and bioethanol synthesis. The findings confirm the practical applicability of the proposed technology for the biotechnological conversion of brewing industry waste into alternative energy sources. Future research should focus on process scaling, genetic modification of bacteria, and the use of mixed microbial cultures to enhance product yields.